tcr β cell signaling technology Search Results


anti tcr β  (Bio-Rad)
88
Bio-Rad anti tcr β
Anti Tcr β, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 88 stars, based on 1 article reviews
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anti–tcr-β (h57-597)  (Becton Dickinson)
90
Becton Dickinson anti–tcr-β (h57-597)
Anti–Tcr β (H57 597), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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percp-cytm5.5 anti-mouse tcr β chain (clone h57-597)  (Becton Dickinson)
90
Becton Dickinson percp-cytm5.5 anti-mouse tcr β chain (clone h57-597)

Percp Cytm5.5 Anti Mouse Tcr β Chain (Clone H57 597), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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tcr β  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology tcr β
Mg2+ regulates glycosylation and plays a unique role in T-cell–mediated immunity, especially in immune protection against EBV. A, flow cytometry histograms of surface expression of NKG2D, CD70, HLA-DR, and CD5 in cycling T cells from HCs cultured either in cRPMI for 5 days, dRPMI for 5 days, or cells that were cultured in dRPMI for 3 days followed by the addition of Mg2+ (0.5 mm) back into the dRPMI for 2 days. B, quantification of the MFI in A. Error bars represent the standard error of the mean of eight independent experiments, and p values were calculated with a paired t test. C, representative Western blot analysis of NKG2D, CD70, <t>TCR-β,</t> and β-actin in cycling T cells from a HC as described in A. Numbers at left indicate kDa standards. Glycosylation patterns are shown at right: fully-glycosylated (2); partially-glycosylated (1); and unglycosylated (0). Data are mean of eight (A and B) or are representative of three (C) independent replicates. D, schematic diagram for killing pathway with NK cells (top) and EBV-721.221(target; bottom) cells. E, flow cytometry analysis of surface expression of NKG2D in EBV-specific NK cells from a HC with cRPMI and dRPMI. F, quantification of the MFI in F. G, percent lysis of autologous EBV-LCLs by EBV-specific NK cells from HC with a different dose of the Mg2+ with significance determined by one-way ANOVA. n indicates the number of independent samples. H, quantification from G. Data are representative of three independent biological replicates. NS, nonsignificant.
Tcr β, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tcr+%CE%B2+cell+signaling+technology/TCR+%CE%B2+Antibody/pmc06746436-621-45-48
Average 90 stars, based on 1 article reviews
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11 - cd3 apc-h7 (clone sk7)  (Becton Dickinson)
90
Becton Dickinson 11 - cd3 apc-h7 (clone sk7)
Mg2+ regulates glycosylation and plays a unique role in T-cell–mediated immunity, especially in immune protection against EBV. A, flow cytometry histograms of surface expression of NKG2D, CD70, HLA-DR, and CD5 in cycling T cells from HCs cultured either in cRPMI for 5 days, dRPMI for 5 days, or cells that were cultured in dRPMI for 3 days followed by the addition of Mg2+ (0.5 mm) back into the dRPMI for 2 days. B, quantification of the MFI in A. Error bars represent the standard error of the mean of eight independent experiments, and p values were calculated with a paired t test. C, representative Western blot analysis of NKG2D, CD70, <t>TCR-β,</t> and β-actin in cycling T cells from a HC as described in A. Numbers at left indicate kDa standards. Glycosylation patterns are shown at right: fully-glycosylated (2); partially-glycosylated (1); and unglycosylated (0). Data are mean of eight (A and B) or are representative of three (C) independent replicates. D, schematic diagram for killing pathway with NK cells (top) and EBV-721.221(target; bottom) cells. E, flow cytometry analysis of surface expression of NKG2D in EBV-specific NK cells from a HC with cRPMI and dRPMI. F, quantification of the MFI in F. G, percent lysis of autologous EBV-LCLs by EBV-specific NK cells from HC with a different dose of the Mg2+ with significance determined by one-way ANOVA. n indicates the number of independent samples. H, quantification from G. Data are representative of three independent biological replicates. NS, nonsignificant.
11 Cd3 Apc H7 (Clone Sk7), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tcr+%CE%B2+cell+signaling+technology/hladr+antibody/10__1158_slash_2326___6066__cir___18___0686-111-12-19
Average 90 stars, based on 1 article reviews
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anti-cd23-pe flow cytometry 1:400  (Becton Dickinson)
90
Becton Dickinson anti-cd23-pe flow cytometry 1:400
Mg2+ regulates glycosylation and plays a unique role in T-cell–mediated immunity, especially in immune protection against EBV. A, flow cytometry histograms of surface expression of NKG2D, CD70, HLA-DR, and CD5 in cycling T cells from HCs cultured either in cRPMI for 5 days, dRPMI for 5 days, or cells that were cultured in dRPMI for 3 days followed by the addition of Mg2+ (0.5 mm) back into the dRPMI for 2 days. B, quantification of the MFI in A. Error bars represent the standard error of the mean of eight independent experiments, and p values were calculated with a paired t test. C, representative Western blot analysis of NKG2D, CD70, <t>TCR-β,</t> and β-actin in cycling T cells from a HC as described in A. Numbers at left indicate kDa standards. Glycosylation patterns are shown at right: fully-glycosylated (2); partially-glycosylated (1); and unglycosylated (0). Data are mean of eight (A and B) or are representative of three (C) independent replicates. D, schematic diagram for killing pathway with NK cells (top) and EBV-721.221(target; bottom) cells. E, flow cytometry analysis of surface expression of NKG2D in EBV-specific NK cells from a HC with cRPMI and dRPMI. F, quantification of the MFI in F. G, percent lysis of autologous EBV-LCLs by EBV-specific NK cells from HC with a different dose of the Mg2+ with significance determined by one-way ANOVA. n indicates the number of independent samples. H, quantification from G. Data are representative of three independent biological replicates. NS, nonsignificant.
Anti Cd23 Pe Flow Cytometry 1:400, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tcr+%CE%B2+cell+signaling+technology/anti+cd23/us09296682-280-116-114
Average 90 stars, based on 1 article reviews
anti-cd23-pe flow cytometry 1:400 - by Bioz Stars, 2026-09
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tcr alpha-beta apc  (Becton Dickinson)
90
Becton Dickinson tcr alpha-beta apc
Mg2+ regulates glycosylation and plays a unique role in T-cell–mediated immunity, especially in immune protection against EBV. A, flow cytometry histograms of surface expression of NKG2D, CD70, HLA-DR, and CD5 in cycling T cells from HCs cultured either in cRPMI for 5 days, dRPMI for 5 days, or cells that were cultured in dRPMI for 3 days followed by the addition of Mg2+ (0.5 mm) back into the dRPMI for 2 days. B, quantification of the MFI in A. Error bars represent the standard error of the mean of eight independent experiments, and p values were calculated with a paired t test. C, representative Western blot analysis of NKG2D, CD70, <t>TCR-β,</t> and β-actin in cycling T cells from a HC as described in A. Numbers at left indicate kDa standards. Glycosylation patterns are shown at right: fully-glycosylated (2); partially-glycosylated (1); and unglycosylated (0). Data are mean of eight (A and B) or are representative of three (C) independent replicates. D, schematic diagram for killing pathway with NK cells (top) and EBV-721.221(target; bottom) cells. E, flow cytometry analysis of surface expression of NKG2D in EBV-specific NK cells from a HC with cRPMI and dRPMI. F, quantification of the MFI in F. G, percent lysis of autologous EBV-LCLs by EBV-specific NK cells from HC with a different dose of the Mg2+ with significance determined by one-way ANOVA. n indicates the number of independent samples. H, quantification from G. Data are representative of three independent biological replicates. NS, nonsignificant.
Tcr Alpha Beta Apc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tcr+%CE%B2+cell+signaling+technology/anti+tcr%CE%B1%CE%B2++t10b9+1a+31/pmc06896983-51-68-73
Average 90 stars, based on 1 article reviews
tcr alpha-beta apc - by Bioz Stars, 2026-09
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t cell receptor beta repertoire tcrß sequencing  (Adaptive Biotechnologies Corp)
86
Adaptive Biotechnologies Corp t cell receptor beta repertoire tcrß sequencing
Mg2+ regulates glycosylation and plays a unique role in T-cell–mediated immunity, especially in immune protection against EBV. A, flow cytometry histograms of surface expression of NKG2D, CD70, HLA-DR, and CD5 in cycling T cells from HCs cultured either in cRPMI for 5 days, dRPMI for 5 days, or cells that were cultured in dRPMI for 3 days followed by the addition of Mg2+ (0.5 mm) back into the dRPMI for 2 days. B, quantification of the MFI in A. Error bars represent the standard error of the mean of eight independent experiments, and p values were calculated with a paired t test. C, representative Western blot analysis of NKG2D, CD70, <t>TCR-β,</t> and β-actin in cycling T cells from a HC as described in A. Numbers at left indicate kDa standards. Glycosylation patterns are shown at right: fully-glycosylated (2); partially-glycosylated (1); and unglycosylated (0). Data are mean of eight (A and B) or are representative of three (C) independent replicates. D, schematic diagram for killing pathway with NK cells (top) and EBV-721.221(target; bottom) cells. E, flow cytometry analysis of surface expression of NKG2D in EBV-specific NK cells from a HC with cRPMI and dRPMI. F, quantification of the MFI in F. G, percent lysis of autologous EBV-LCLs by EBV-specific NK cells from HC with a different dose of the Mg2+ with significance determined by one-way ANOVA. n indicates the number of independent samples. H, quantification from G. Data are representative of three independent biological replicates. NS, nonsignificant.
T Cell Receptor Beta Repertoire Tcrß Sequencing, supplied by Adaptive Biotechnologies Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tcr+%CE%B2+cell+signaling+technology/beta+cell+receptor+repertoire+sequencing+t+tcr%C3%9F/10__1016_slash_j__jtho__2019__08__911-7-39-46
Average 86 stars, based on 1 article reviews
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anti tcr b percp cy5 5  (Cytek Biosciences)
93
Cytek Biosciences anti tcr b percp cy5 5
Mg2+ regulates glycosylation and plays a unique role in T-cell–mediated immunity, especially in immune protection against EBV. A, flow cytometry histograms of surface expression of NKG2D, CD70, HLA-DR, and CD5 in cycling T cells from HCs cultured either in cRPMI for 5 days, dRPMI for 5 days, or cells that were cultured in dRPMI for 3 days followed by the addition of Mg2+ (0.5 mm) back into the dRPMI for 2 days. B, quantification of the MFI in A. Error bars represent the standard error of the mean of eight independent experiments, and p values were calculated with a paired t test. C, representative Western blot analysis of NKG2D, CD70, <t>TCR-β,</t> and β-actin in cycling T cells from a HC as described in A. Numbers at left indicate kDa standards. Glycosylation patterns are shown at right: fully-glycosylated (2); partially-glycosylated (1); and unglycosylated (0). Data are mean of eight (A and B) or are representative of three (C) independent replicates. D, schematic diagram for killing pathway with NK cells (top) and EBV-721.221(target; bottom) cells. E, flow cytometry analysis of surface expression of NKG2D in EBV-specific NK cells from a HC with cRPMI and dRPMI. F, quantification of the MFI in F. G, percent lysis of autologous EBV-LCLs by EBV-specific NK cells from HC with a different dose of the Mg2+ with significance determined by one-way ANOVA. n indicates the number of independent samples. H, quantification from G. Data are representative of three independent biological replicates. NS, nonsignificant.
Anti Tcr B Percp Cy5 5, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tcr+%CE%B2+cell+signaling+technology/PerCP-Cyanine5%2E5+Anti-Mouse+TCR+beta/pmc09131289-6-0-2
Average 93 stars, based on 1 article reviews
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easysep human tcr alpha/beta depletion kit  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc easysep human tcr alpha/beta depletion kit
Mg2+ regulates glycosylation and plays a unique role in T-cell–mediated immunity, especially in immune protection against EBV. A, flow cytometry histograms of surface expression of NKG2D, CD70, HLA-DR, and CD5 in cycling T cells from HCs cultured either in cRPMI for 5 days, dRPMI for 5 days, or cells that were cultured in dRPMI for 3 days followed by the addition of Mg2+ (0.5 mm) back into the dRPMI for 2 days. B, quantification of the MFI in A. Error bars represent the standard error of the mean of eight independent experiments, and p values were calculated with a paired t test. C, representative Western blot analysis of NKG2D, CD70, <t>TCR-β,</t> and β-actin in cycling T cells from a HC as described in A. Numbers at left indicate kDa standards. Glycosylation patterns are shown at right: fully-glycosylated (2); partially-glycosylated (1); and unglycosylated (0). Data are mean of eight (A and B) or are representative of three (C) independent replicates. D, schematic diagram for killing pathway with NK cells (top) and EBV-721.221(target; bottom) cells. E, flow cytometry analysis of surface expression of NKG2D in EBV-specific NK cells from a HC with cRPMI and dRPMI. F, quantification of the MFI in F. G, percent lysis of autologous EBV-LCLs by EBV-specific NK cells from HC with a different dose of the Mg2+ with significance determined by one-way ANOVA. n indicates the number of independent samples. H, quantification from G. Data are representative of three independent biological replicates. NS, nonsignificant.
Easysep Human Tcr Alpha/Beta Depletion Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tcr+%CE%B2+cell+signaling+technology/easysep+human+tcr+alpha+beta+depletion+kit/pmc11208159-359-10-16
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allophycocyanin-labeled anti-tcr β  (Becton Dickinson)
90
Becton Dickinson allophycocyanin-labeled anti-tcr β
Mg2+ regulates glycosylation and plays a unique role in T-cell–mediated immunity, especially in immune protection against EBV. A, flow cytometry histograms of surface expression of NKG2D, CD70, HLA-DR, and CD5 in cycling T cells from HCs cultured either in cRPMI for 5 days, dRPMI for 5 days, or cells that were cultured in dRPMI for 3 days followed by the addition of Mg2+ (0.5 mm) back into the dRPMI for 2 days. B, quantification of the MFI in A. Error bars represent the standard error of the mean of eight independent experiments, and p values were calculated with a paired t test. C, representative Western blot analysis of NKG2D, CD70, <t>TCR-β,</t> and β-actin in cycling T cells from a HC as described in A. Numbers at left indicate kDa standards. Glycosylation patterns are shown at right: fully-glycosylated (2); partially-glycosylated (1); and unglycosylated (0). Data are mean of eight (A and B) or are representative of three (C) independent replicates. D, schematic diagram for killing pathway with NK cells (top) and EBV-721.221(target; bottom) cells. E, flow cytometry analysis of surface expression of NKG2D in EBV-specific NK cells from a HC with cRPMI and dRPMI. F, quantification of the MFI in F. G, percent lysis of autologous EBV-LCLs by EBV-specific NK cells from HC with a different dose of the Mg2+ with significance determined by one-way ANOVA. n indicates the number of independent samples. H, quantification from G. Data are representative of three independent biological replicates. NS, nonsignificant.
Allophycocyanin Labeled Anti Tcr β, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tcr+%CE%B2+cell+signaling+technology/allophycocyanin+labeled+anti+tcr+%CE%B2/pmc02291538-169-33-39
Average 90 stars, based on 1 article reviews
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anti–tcr-β (h57-597, hamster igg)  (Becton Dickinson)
90
Becton Dickinson anti–tcr-β (h57-597, hamster igg)
Features of <t>TCR-dependent</t> apoptosis of CD4 + 8 + and HSA hi CD4 + 8 − thymocyte subsets in vitro. Purified TCR lo CD4 + 8 + or HSA hi CD4 + 8 − thymocytes from B6 or B6 lpr/lpr mice were cultured for 20 h in vitro in 200-μl wells coated <t>with</t> <t>anti-TCR</t> <t>(H57-597)</t> mAb at 0.1 or 10 μg/ml plus/minus anti-CD28 (37.51) mAb at 20 μg/ml; as controls, cells were cultured in medium alone or in wells coated with anti-CD28 mAb alone. Apoptosis was detected by TUNEL staining and FACS™ analysis. (A) Representative TUNEL staining versus forward scatter (FSC) for CD4 + 8 + cells cultured in medium alone or in wells coated with a mixture of anti-TCR (0.1 μg/ml) and anti-CD28 mAbs; the numbers in the panels show the proportion of TUNEL + cells. (B) Difference in (Δ) apoptosis levels (%) for CD4 + 8 + and HSA hi CD4 + 8 − cells cultured with anti-TCR mAb (0.1 or 10 μg/ml) alone or with anti-TCR plus anti-CD28 mAbs. The data show mean percent differences in apoptosis for triplicate cultures.
Anti–Tcr β (H57 597, Hamster Igg), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tcr+%CE%B2+cell+signaling+technology/annexin+v/pmc02195556-8-7-6
Average 90 stars, based on 1 article reviews
anti–tcr-β (h57-597, hamster igg) - by Bioz Stars, 2026-09
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Image Search Results


Journal: Cell reports

Article Title: Airway Microbiota-Host Interactions Regulate Secretory Leukocyte Protease Inhibitor Levels and Influence Allergic Airway Inflammation

doi: 10.1016/j.celrep.2020.108331

Figure Lengend Snippet:

Article Snippet: PerCP-CyTM5.5 anti-mouse TCR β chain (Clone H57-597) , BD PharmigenTM , Cat# 560657; RRID: AB_1727575.

Techniques: Purification, Control, Virus, Isolation, Recombinant, Adjuvant, Saline, Electron Microscopy, Enzyme-linked Immunosorbent Assay, Qubit Protein Assay, Selection, Reverse Transcription, Staining, Sequencing, Expressing, Software

Mg2+ regulates glycosylation and plays a unique role in T-cell–mediated immunity, especially in immune protection against EBV. A, flow cytometry histograms of surface expression of NKG2D, CD70, HLA-DR, and CD5 in cycling T cells from HCs cultured either in cRPMI for 5 days, dRPMI for 5 days, or cells that were cultured in dRPMI for 3 days followed by the addition of Mg2+ (0.5 mm) back into the dRPMI for 2 days. B, quantification of the MFI in A. Error bars represent the standard error of the mean of eight independent experiments, and p values were calculated with a paired t test. C, representative Western blot analysis of NKG2D, CD70, TCR-β, and β-actin in cycling T cells from a HC as described in A. Numbers at left indicate kDa standards. Glycosylation patterns are shown at right: fully-glycosylated (2); partially-glycosylated (1); and unglycosylated (0). Data are mean of eight (A and B) or are representative of three (C) independent replicates. D, schematic diagram for killing pathway with NK cells (top) and EBV-721.221(target; bottom) cells. E, flow cytometry analysis of surface expression of NKG2D in EBV-specific NK cells from a HC with cRPMI and dRPMI. F, quantification of the MFI in F. G, percent lysis of autologous EBV-LCLs by EBV-specific NK cells from HC with a different dose of the Mg2+ with significance determined by one-way ANOVA. n indicates the number of independent samples. H, quantification from G. Data are representative of three independent biological replicates. NS, nonsignificant.

Journal: The Journal of Biological Chemistry

Article Title: Magnesium transporter 1 (MAGT1) deficiency causes selective defects in N- linked glycosylation and expression of immune-response genes

doi: 10.1074/jbc.RA119.008903

Figure Lengend Snippet: Mg2+ regulates glycosylation and plays a unique role in T-cell–mediated immunity, especially in immune protection against EBV. A, flow cytometry histograms of surface expression of NKG2D, CD70, HLA-DR, and CD5 in cycling T cells from HCs cultured either in cRPMI for 5 days, dRPMI for 5 days, or cells that were cultured in dRPMI for 3 days followed by the addition of Mg2+ (0.5 mm) back into the dRPMI for 2 days. B, quantification of the MFI in A. Error bars represent the standard error of the mean of eight independent experiments, and p values were calculated with a paired t test. C, representative Western blot analysis of NKG2D, CD70, TCR-β, and β-actin in cycling T cells from a HC as described in A. Numbers at left indicate kDa standards. Glycosylation patterns are shown at right: fully-glycosylated (2); partially-glycosylated (1); and unglycosylated (0). Data are mean of eight (A and B) or are representative of three (C) independent replicates. D, schematic diagram for killing pathway with NK cells (top) and EBV-721.221(target; bottom) cells. E, flow cytometry analysis of surface expression of NKG2D in EBV-specific NK cells from a HC with cRPMI and dRPMI. F, quantification of the MFI in F. G, percent lysis of autologous EBV-LCLs by EBV-specific NK cells from HC with a different dose of the Mg2+ with significance determined by one-way ANOVA. n indicates the number of independent samples. H, quantification from G. Data are representative of three independent biological replicates. NS, nonsignificant.

Article Snippet: The following antibodies were used: MAGT1 (Mer catalog no. 17-1, 1:50 dilution, produced by Merck); TUSC3 (Mer 85 catalog no. 38-1, affinity-purified, produced by Merck) ( Fig. S6 ) 8 ; β-actin (ab20272, Abcam); NKG2D (D-20, sc-9621, Santa Cruz Biotechnology); tubulin (AA2, 05-661, Merck Millipore); TCR-β (H-197, sc-9101, Santa Cruz Biotechnology); CERS2 (A303-193A, Bethyl Laboratories, Inc.); ribophorin I (C-15, sc-12164, Santa Cruz Biotechnology); COP1 (PA1-061, Thermo Fisher Scientific); EEA1 (C45B10, 3288 Cell Signaling); STT3B (HPA036646, Sigma); OST 48 (Santa Cruz Biotechnology); Na,K-ATPase α1 (D4Y7E, 23565, Cell Signaling).

Techniques: Glycoproteomics, Flow Cytometry, Expressing, Cell Culture, Western Blot, Lysis

Features of TCR-dependent apoptosis of CD4 + 8 + and HSA hi CD4 + 8 − thymocyte subsets in vitro. Purified TCR lo CD4 + 8 + or HSA hi CD4 + 8 − thymocytes from B6 or B6 lpr/lpr mice were cultured for 20 h in vitro in 200-μl wells coated with anti-TCR (H57-597) mAb at 0.1 or 10 μg/ml plus/minus anti-CD28 (37.51) mAb at 20 μg/ml; as controls, cells were cultured in medium alone or in wells coated with anti-CD28 mAb alone. Apoptosis was detected by TUNEL staining and FACS™ analysis. (A) Representative TUNEL staining versus forward scatter (FSC) for CD4 + 8 + cells cultured in medium alone or in wells coated with a mixture of anti-TCR (0.1 μg/ml) and anti-CD28 mAbs; the numbers in the panels show the proportion of TUNEL + cells. (B) Difference in (Δ) apoptosis levels (%) for CD4 + 8 + and HSA hi CD4 + 8 − cells cultured with anti-TCR mAb (0.1 or 10 μg/ml) alone or with anti-TCR plus anti-CD28 mAbs. The data show mean percent differences in apoptosis for triplicate cultures.

Journal: The Journal of Experimental Medicine

Article Title: Several Different Cell Surface Molecules Control Negative Selection of Medullary Thymocytes

doi:

Figure Lengend Snippet: Features of TCR-dependent apoptosis of CD4 + 8 + and HSA hi CD4 + 8 − thymocyte subsets in vitro. Purified TCR lo CD4 + 8 + or HSA hi CD4 + 8 − thymocytes from B6 or B6 lpr/lpr mice were cultured for 20 h in vitro in 200-μl wells coated with anti-TCR (H57-597) mAb at 0.1 or 10 μg/ml plus/minus anti-CD28 (37.51) mAb at 20 μg/ml; as controls, cells were cultured in medium alone or in wells coated with anti-CD28 mAb alone. Apoptosis was detected by TUNEL staining and FACS™ analysis. (A) Representative TUNEL staining versus forward scatter (FSC) for CD4 + 8 + cells cultured in medium alone or in wells coated with a mixture of anti-TCR (0.1 μg/ml) and anti-CD28 mAbs; the numbers in the panels show the proportion of TUNEL + cells. (B) Difference in (Δ) apoptosis levels (%) for CD4 + 8 + and HSA hi CD4 + 8 − cells cultured with anti-TCR mAb (0.1 or 10 μg/ml) alone or with anti-TCR plus anti-CD28 mAbs. The data show mean percent differences in apoptosis for triplicate cultures.

Article Snippet: The following mAbs were purchased from PharMingen: anti–TCR-β (H57-597, hamster IgG), anti-CD2 (RM2-5, rat IgG), anti-CD5 (53-7.3, rat IgG), anti–LFA-1 (CD11) (M17/4, rat IgG), anti-CD27 (LG.3A10, hamster IgG), anti-CD28 (37.51, hamster IgG), anti-CD40L (CD154, MR1, hamster IgG), anti-CD43 (S7, rat IgG), anti-CD48 (HM48-1, hamster IgG), anti-CD49d (very late antigen [VLA]-4; R1-2, rat IgG), anti-CD81 (2F7, hamster IgG), anti–thymic shared antigen (TSA)-1 (MTS35, rat IgG), anti–CTL-associated antigen (CTLA)-4 (CD152; UC10-4F10-11, hamster IgG), anti-CD95 (Fas; Jo2, hamster IgG), and Cy-Chrome–conjugated anti-CD4 (H129.19, rat IgG).

Techniques: In Vitro, Purification, Cell Culture, TUNEL Assay, Staining

Capacity of various cell surface molecules on thymocytes to provide costimulation for TCR-dependent apoptosis of CD4 + 8 + and HSA hi CD4 + 8 − thymocytes in vitro. Purified subsets of thymocytes from normal B6 mice were cultured overnight with anti-TCR mAb at 0.1 or 10 μg/ml plus/minus the mAbs shown (all at 20 μg/ml). The data represent mean percent differences in (Δ) apoptosis for triplicate cultures. The concentrations of anti-TCR mAbs used are shown in the panels.

Journal: The Journal of Experimental Medicine

Article Title: Several Different Cell Surface Molecules Control Negative Selection of Medullary Thymocytes

doi:

Figure Lengend Snippet: Capacity of various cell surface molecules on thymocytes to provide costimulation for TCR-dependent apoptosis of CD4 + 8 + and HSA hi CD4 + 8 − thymocytes in vitro. Purified subsets of thymocytes from normal B6 mice were cultured overnight with anti-TCR mAb at 0.1 or 10 μg/ml plus/minus the mAbs shown (all at 20 μg/ml). The data represent mean percent differences in (Δ) apoptosis for triplicate cultures. The concentrations of anti-TCR mAbs used are shown in the panels.

Article Snippet: The following mAbs were purchased from PharMingen: anti–TCR-β (H57-597, hamster IgG), anti-CD2 (RM2-5, rat IgG), anti-CD5 (53-7.3, rat IgG), anti–LFA-1 (CD11) (M17/4, rat IgG), anti-CD27 (LG.3A10, hamster IgG), anti-CD28 (37.51, hamster IgG), anti-CD40L (CD154, MR1, hamster IgG), anti-CD43 (S7, rat IgG), anti-CD48 (HM48-1, hamster IgG), anti-CD49d (very late antigen [VLA]-4; R1-2, rat IgG), anti-CD81 (2F7, hamster IgG), anti–thymic shared antigen (TSA)-1 (MTS35, rat IgG), anti–CTL-associated antigen (CTLA)-4 (CD152; UC10-4F10-11, hamster IgG), anti-CD95 (Fas; Jo2, hamster IgG), and Cy-Chrome–conjugated anti-CD4 (H129.19, rat IgG).

Techniques: In Vitro, Purification, Cell Culture

For CD28, CD5, and CD43, costimulation for Fas-independent apoptosis of HSA hi CD4 + 8 − cells in vitro fails with strong TCR ligation. HSA hi CD4 + 8 − cells from normal B6 or B6 lpr/lpr mice were cultured overnight with either a low (0.1 μg/ml; left) or high (10 μg/ml; right) concentration of anti-TCR mAb plus/minus anti-CD5, anti-CD28, or anti-CD43 mAbs (20 μg/ml). The data show mean percent differences in (Δ) apoptosis for triplicate cultures.

Journal: The Journal of Experimental Medicine

Article Title: Several Different Cell Surface Molecules Control Negative Selection of Medullary Thymocytes

doi:

Figure Lengend Snippet: For CD28, CD5, and CD43, costimulation for Fas-independent apoptosis of HSA hi CD4 + 8 − cells in vitro fails with strong TCR ligation. HSA hi CD4 + 8 − cells from normal B6 or B6 lpr/lpr mice were cultured overnight with either a low (0.1 μg/ml; left) or high (10 μg/ml; right) concentration of anti-TCR mAb plus/minus anti-CD5, anti-CD28, or anti-CD43 mAbs (20 μg/ml). The data show mean percent differences in (Δ) apoptosis for triplicate cultures.

Article Snippet: The following mAbs were purchased from PharMingen: anti–TCR-β (H57-597, hamster IgG), anti-CD2 (RM2-5, rat IgG), anti-CD5 (53-7.3, rat IgG), anti–LFA-1 (CD11) (M17/4, rat IgG), anti-CD27 (LG.3A10, hamster IgG), anti-CD28 (37.51, hamster IgG), anti-CD40L (CD154, MR1, hamster IgG), anti-CD43 (S7, rat IgG), anti-CD48 (HM48-1, hamster IgG), anti-CD49d (very late antigen [VLA]-4; R1-2, rat IgG), anti-CD81 (2F7, hamster IgG), anti–thymic shared antigen (TSA)-1 (MTS35, rat IgG), anti–CTL-associated antigen (CTLA)-4 (CD152; UC10-4F10-11, hamster IgG), anti-CD95 (Fas; Jo2, hamster IgG), and Cy-Chrome–conjugated anti-CD4 (H129.19, rat IgG).

Techniques: In Vitro, Ligation, Cell Culture, Concentration Assay

Capacity of cytokines to inhibit Fas-independent apoptosis of thymocyte subsets in vitro. Purified CD4 + 8 + or HSA hi CD4 + 8 − thymocytes from normal B6 mice were cultured overnight either in medium alone (gray bars) or with a mixture of anti-TCR (0.1 μg/ml) and anti-CD28 mAbs (black bars); where indicated, the recombinant cytokines shown were added to the cultures at 100 U/ml. The data show mean percent apoptosis (top panels) and percent difference in (Δ) apoptosis (bottom panels).

Journal: The Journal of Experimental Medicine

Article Title: Several Different Cell Surface Molecules Control Negative Selection of Medullary Thymocytes

doi:

Figure Lengend Snippet: Capacity of cytokines to inhibit Fas-independent apoptosis of thymocyte subsets in vitro. Purified CD4 + 8 + or HSA hi CD4 + 8 − thymocytes from normal B6 mice were cultured overnight either in medium alone (gray bars) or with a mixture of anti-TCR (0.1 μg/ml) and anti-CD28 mAbs (black bars); where indicated, the recombinant cytokines shown were added to the cultures at 100 U/ml. The data show mean percent apoptosis (top panels) and percent difference in (Δ) apoptosis (bottom panels).

Article Snippet: The following mAbs were purchased from PharMingen: anti–TCR-β (H57-597, hamster IgG), anti-CD2 (RM2-5, rat IgG), anti-CD5 (53-7.3, rat IgG), anti–LFA-1 (CD11) (M17/4, rat IgG), anti-CD27 (LG.3A10, hamster IgG), anti-CD28 (37.51, hamster IgG), anti-CD40L (CD154, MR1, hamster IgG), anti-CD43 (S7, rat IgG), anti-CD48 (HM48-1, hamster IgG), anti-CD49d (very late antigen [VLA]-4; R1-2, rat IgG), anti-CD81 (2F7, hamster IgG), anti–thymic shared antigen (TSA)-1 (MTS35, rat IgG), anti–CTL-associated antigen (CTLA)-4 (CD152; UC10-4F10-11, hamster IgG), anti-CD95 (Fas; Jo2, hamster IgG), and Cy-Chrome–conjugated anti-CD4 (H129.19, rat IgG).

Techniques: In Vitro, Purification, Cell Culture, Recombinant

Costimulation-dependent apoptosis induced by TCR/CD28, TCR/CD5, or TCR/CD43 ligation in vitro is abolished by IL-7. Purified HSA hi CD4 + 8 − thymocytes from B6 lpr/lpr mice were cultured with either a low or high concentration of anti-TCR mAb plus/minus mAbs specific for CD28, CD5, or CD43; where indicated, IL-7 was added to the cultures at 100 U/ml. The data show mean levels of difference in (Δ) apoptosis for triplicate cultures. Gray bar, anti-TCR (0.1 μg/ml); dotted bar, anti-TCR (0.1 μg/ml) plus IL-7; black bar, anti-TCR (10 μg/ml).

Journal: The Journal of Experimental Medicine

Article Title: Several Different Cell Surface Molecules Control Negative Selection of Medullary Thymocytes

doi:

Figure Lengend Snippet: Costimulation-dependent apoptosis induced by TCR/CD28, TCR/CD5, or TCR/CD43 ligation in vitro is abolished by IL-7. Purified HSA hi CD4 + 8 − thymocytes from B6 lpr/lpr mice were cultured with either a low or high concentration of anti-TCR mAb plus/minus mAbs specific for CD28, CD5, or CD43; where indicated, IL-7 was added to the cultures at 100 U/ml. The data show mean levels of difference in (Δ) apoptosis for triplicate cultures. Gray bar, anti-TCR (0.1 μg/ml); dotted bar, anti-TCR (0.1 μg/ml) plus IL-7; black bar, anti-TCR (10 μg/ml).

Article Snippet: The following mAbs were purchased from PharMingen: anti–TCR-β (H57-597, hamster IgG), anti-CD2 (RM2-5, rat IgG), anti-CD5 (53-7.3, rat IgG), anti–LFA-1 (CD11) (M17/4, rat IgG), anti-CD27 (LG.3A10, hamster IgG), anti-CD28 (37.51, hamster IgG), anti-CD40L (CD154, MR1, hamster IgG), anti-CD43 (S7, rat IgG), anti-CD48 (HM48-1, hamster IgG), anti-CD49d (very late antigen [VLA]-4; R1-2, rat IgG), anti-CD81 (2F7, hamster IgG), anti–thymic shared antigen (TSA)-1 (MTS35, rat IgG), anti–CTL-associated antigen (CTLA)-4 (CD152; UC10-4F10-11, hamster IgG), anti-CD95 (Fas; Jo2, hamster IgG), and Cy-Chrome–conjugated anti-CD4 (H129.19, rat IgG).

Techniques: Ligation, In Vitro, Purification, Cell Culture, Concentration Assay